Delphinidin, Luteolin and Halogenated Boroxine Modulate CAT Gene Expression in Cultured Lymphocytes

Luteolin and delphinidin are the flavonoids with known protective roles. They inhibit genotoxic effects induced by halogenated boroxine (HB) in vitro. Statistically significant decrease frequency of micronuclei nuclear buds suppression occurrence aberrant cells were observed before, but mechanism its anti-genotoxic activity is still not clear. In our experiment we aimed to quantify HB on relative expression CAT (catalase) gene explore antioxidative luteolin via restoration activity. Cell cultures from peripheral blood lymphocytes five healthy donors established treated independent concomitant treatments or delphinidin. Total RNA was isolated harvested reverse-transcribed. SYBR based Real-Time PCR amplification method used. Analysis results included normalization ratio target (CAT) housekeeping (GAPDH) statistical analysis (REST®). itself lead downregulation as well HB. But simultaneous treatment bioflavonoids upregulation. Delphinidin caused upregulation expression. Obtained may indicate role oxidative damage HB, also that new approaches applications should include monitoring corresponding antioxidant system. Our findings there a quantifiable effect delphinidine genes which could be used exact stress related events.